�ber die Cellobiose

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Synthesis of [C]Cellobiose with Clostridium thermocellum Cellobiose Phosphorylase.

Cellobiose labeled either at the reducing end (4-O-beta-d-glucopyranosyl-d-[U-C]glucopyranose) or at the nonreducing end (4-O-beta-d-[U-C]glucopyranosyl-d -glucopyranose) was synthesized with Clostridium thermocellum cellobiose phosphorylase at greater than 98% purity. The radioactive cellobioses were identical to authentic cellobiose in melting point, optical rotation, isotopic dilution, and c...

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Characteristics of cellobiose phosphorylase.

The phosphorolysis of cellobiose was first demonstrated by Sih and McBee (1955a, b). These workers found that cellobiose phosphorylase was present in cell-free extracts of Clostridium thermocellum. Recently cellobiose phosphorylase has been reported from two other bacteria, Ruminococcus flavefaciens (Ayers, 1958, 1959), and Cellvibrio gilvus (Hulcher and King, 1958). This enzyme also catalyzes ...

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Cellobiose Dehydrogenase Production by the Genus Cladosporium

Cellobiose dehydrogenase (CDH EC.1.1.5.1) is an extracellular enzyme that mainly produced by wood-degrading fungi. It oxidizes cellobiose to cellobionolactone using a wide spectrum of electron acceptors. The key roles of CDH in growth, metabolism, and some other important cellular processes such as cellulose degradation in fungi have been noted. Since the demands for finding new sources of CDH ...

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Cellobiose metabolism in Aerobacter aerogenes. 3. Cleavage of cellobiose monophosphate by a phospho- -glucosidase.

An enzyme in extracts of Aerobacter aerogenes was shown to catalyze the hydrolytic cleavage of cellobiose monophosphate (6-0-phosphoryl-b-D-ghtcopyranosyl-(1 --f 4)-D-gillcase) to yield equimolar amounts of D-glucose and D-glucose 6-phosphate. The enzyme was partially purified and characterized. In addition to cellobiose monophosphate, it catalyzed the cleavage of cellobiitol phosphate, arbutin...

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Overcoming inefficient cellobiose fermentation by cellobiose phosphorylase in the presence of xylose

BACKGROUND Cellobiose and xylose co-fermentation holds promise for efficiently producing biofuels from plant biomass. Cellobiose phosphorylase (CBP), an intracellular enzyme generally found in anaerobic bacteria, cleaves cellobiose to glucose and glucose-1-phosphate, providing energetic advantages under the anaerobic conditions required for large-scale biofuel production. However, the efficienc...

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ژورنال

عنوان ژورنال: Monatshefte f�r Chemie

سال: 1901

ISSN: 0026-9247,1434-4475

DOI: 10.1007/bf01530313